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The lipid with the fish roe possibly can have an impact on the accessibility of proteases to proteins. Fish roe by using a higher lipid content may very well be considerably less proficiently hydrolyzed due to hindering result with the Extra fat molecules, which Restrict the protease use of the protein. Fatty acids may perhaps bind to proteins and alter their three-dimensional construction, which affects the proteases’ interaction with proteins [37,38]. One more reason that can demonstrate the noticed variances within the hydrolysis potential of your 3 proteases is their hydrolytic specificity. The protease source as well as the substrate used for the hydrolysis method can effects the rate along with the extent with the hydrolysis process. For example, Kumar et al. [39] received trypsin from 3 distinctive fish Catla catla, Labeo rohit, Hypophthalmichthys molitrix had been accustomed to hydrolysis casein, soybean food, silver carp, and Chilean fish meal and the result demonstrated that the DH is affected via the enzyme resource and substrate composition [39].
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